Penicillin-binding proteins of Streptomyces cacaoi, Streptomyces olivaceus, and Streptomyces clavuligerus
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چکیده
منابع مشابه
Mechanisms of acquired penicillin-resistance in Streptomyces cacaoi. Role of penicillin-binding proteins in penicillin resistant mutants.
Several mutants isolated after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis of Streptomyces cacaoi strain KCCS-0352, were resistant to benzylpenicillin ranging in concentration from 1,000 to 5,000 micrograms/ml (4- to 20-fold more resistant than the parent). These mutants also acquired resistance to mecillinam, cephamycin C and methicillin. The affinity for beta-lactams of penicillin-bindin...
متن کاملVisualization of penicillin-binding proteins during sporulation of Streptomyces griseus.
We used fluorescein-tagged beta-lactam antibiotics to visualize penicillin-binding proteins (PBPs) in sporulating cultures of Streptomyces griseus. Six PBPs were identified in membranes prepared from growing and sporulating cultures. The binding activity of an 85-kDa PBP increased fourfold by 10 to 12 h of sporulation, at which time the sporulation septa were formed. Cefoxitin inhibited the int...
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Clavulanic acid is produced industrially by fermentation of Streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant DNA technology, and media composition and growth condition optimization. The main objective of this study was to increase the level of clavulanic acid production from Streptomyces clavuligerus (DSM 738), using UV irradiation. After...
متن کاملThe active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G.
The active-site serine of the extracellular beta-lactamases of Streptomyces cacaoi and Streptomyces albus G has been labelled with beta-iodopenicillanate. The determination of the sequence of the labelled peptides obtained after trypsin digestion of the denatured proteins indicate both enzymes to be class A beta-lactamases. Surprisingly the two Streptomyces enzymes do not appear to be especiall...
متن کاملOverproduction of Clavulanic Acid by UV Mutagenesis of Streptomyces clavuligerus
Clavulanic acid is produced industrially by fermentation of Streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant DNA technology, and media composition and growth condition optimization. The main objective of this study was to increase the level of clavulanic acid production from Streptomyces clavuligerus (DSM 738), using UV irradiation. After...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1980
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.17.1.1